Part:BBa_I769106:Design

ech protein generator for vanillin biosynthesis

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 892
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 892
  Illegal NotI site found at 836
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 892
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 892
  Illegal NgoMIV site found at 265
  Illegal NgoMIV site found at 283
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Needs to be used in conjunction with fcs in order to generate vanillin.

Source

Uses BBa_I769105 as well as a promoter, RBS, and terminator. Uses IPTG inducible promoter in order to test construct. Contains high efficiency RBS and terminator.